The invention discloses a harmless fabrication method of sturgeon taxidermy specimen. The harmless fabrication method comprises the following steps: A. stripping fish skin: dissecting the part from the pelvic fin to the anus along the centerline of the belly of a fish, and removing internal organs, muscles and the like; B. infusing and degreasing: moving the stripped fish skin together with the head into an infusing container, soaking the fish skin and the head by high-concentration alcohol, changing the alcohol at regular intervals, and conducting harmless treatment on liquid waste; C. conducting antiseptic treatment: arranging the soaked fish in clear water for softening, coating antiseptic miscellaneous reagents for fish specimens inside and outside the fish skin and the head; D. filling and sewing: taking a tree trunk as a support shaft rod so as to enable the fish skin to be stretched completely, and using nylon threads to sew the cut of the specimen; and E. shaping and displaying: fixing the fin rays of the specimen by a clamping plate, naturally drying the fin rays by air, filling the gill, installing ocular prosthesis, coating liquid paraffin on the whole specimen, and fixing the specimen on a display stand. The method is easy to implement, the operation is convenient, the specimen is infused and degreased thoroughly, the harmless reagent is long in antiseptic effect, and the specimen fabricated by the method is safe and environment-friendly.