A digital holography imaging system with speckle removing effect by multiple overlaying belongs to the field of digital holography. An emergent end of a laser is provided with an optical fiber coupler, the optical fiber coupler is connected with an optical fiber splitter, and two optical fibers from the optical fiber splitter are respectively connected to a two-dimension electric translation stage and an optical fiber clamp. Emergent light of the optical fiber connected to the two-dimension electric translation stage is perpendicular to the two-dimension electric translation stage and moves along with the same horizontally, a lens and a sample are disposed along the emergent direction of the emergent light, and the connecting line of the center of the sample and the center of a combiner crystal is perpendicular to one side of the combiner crystal. Emergent light of the optical fiber connected with the optical fiber clamp is perpendicular to the other side of the combiner crystal, the side is perpendicular to the vertical side of the center of the sample, a CCD (charge-coupled device) camera is arranged on the side opposite to the side of the combiner crystal which is perpendicular to the center of the sample, and is connected with the two-dimension electric translation stage and a computer. The digital holography imaging system with the speckle removing effect by multiple overlaying has the advantages that speckle noise of digital holography reproduction image is reduced and signal to noise ratio is increased.