The invention discloses a method for detecting a nicotine release behavior of a gum base type smoke-free tobacco product. The method for detecting the nicotine release behavior of the gum base type smoke-free tobacco product is characterized by being capable of simulating the release condition of nicotine in the gum base type tobacco product when the tobacco product is chewed in the mouth of a person and performing real-time and quantitative detection on a release amount and a release speed. The method comprises the specific steps of: (1) releasing and collecting nicotine in vitro; (2) performing quantitative analysis on the content of the nicotine in collected liquid and residual samples; and (3) drawing an in-vitro nicotine release curve. The method can be used for detecting the nicotine release speed and the nicotine release degree of the gum base type tobacco product so as to provide the technical support for researching and judging the safety of the gum base type smoke-free tobacco product and performing quality regulation and control. The method is close to the true condition of the gum base type smoke-free tobacco product consumed by the person, is rapid and exact, good in repeatability and short in detection period, is suitable for detection of the gum base type smoke-free tobacco product in various sizes and is convenient to popularize.