A root-limiting direct-sowing tobacco seedling tray and a root-limiting direct-sowing tobacco seedling raising method belong to the field of plant cultivation. The seedling tray comprises a tray body, a plurality of seedling cells are evenly distributed on the tray body, each seedling cell is in the shape of an inverted cone, and a water control hole is reserved on the lower middle of the lateralconical side of each seedling cell. The root-limiting direct-sowing tobacco seedling raising method includes direct sowing with the tray, separating the seedling tray from seedbed soil, applying controlled release fertilizer once, and supply atomized water to the upper portion of the seedling tray. The method used with chemical and intermittent water supply and seedling exercising techniques has the advantages that leaf trimming frequency in seedling raising is reduced, down-penetration of root systems is controlled, the length of main roots is increased, amount of adventitious roots is reduced, damage of tobacco seedlings in transplant is reduced, possibility of invasion of rhizome pathogens is lowered, intone resultant of the transplanted tobacco is lowered, and production quality and harmony of internal chemical compositions of tobacco leaves are improved.