The invention provides porous graphene with a stratified columnar support structure and its preparation method. The method comprises: (1) adding a porous metal oxide inorganic template with a lamellar structure and a precursor of carbon into an organic solvent able to dissolve the carbon precursor; (2) heating and vacuum pumping the product of step (1) so as to remove the solvent; (3) subjecting the product of step (2) to heat treatment in an inert atmosphere for 30-400min at a temperature of 500-1000DEG C; then collecting the solid product after cooling; (4) treating the solid product with a dilute acid for removing the metal oxide template, thus obtaining a porous graphene material. The porous graphene material with a stratified columnar support structure prepared by the preparation method of the invention not only has holes with a diameter larger than 2nm distributed in the graphene lamellar layer, but also has columnar supports between graphene lamellas. Able to reduce the mass transfer resistance between graphene lamellas, the porous graphene in the invention boasts wide application prospects in the fields of energy storage, hydrogen storage, catalysis, and environmental protection, etc.