The invention relates to a tissue-specific promoter and its application. The nucleotide sequence of the tissue-specific promoter contains a sequence of (a) or (b) as follows: (a) a nucleotide sequence which is shown from the 16th nucleotide to the 2310th nucleotide of SEQ ID NO:1, and also comprises the promoter function; and (b) a nucleotide sequence which hybridizes with the limited nucleotide sequence from (a). The tissue-specific promoter provided by the invention is firstly isolated from PEPC gene of Chinese maize variety Z31. In the meanwhile, the promoter obviously increases the expression level of target heterologous nucleotide sequences in plant photosynthetic tissues, but obviously reduces the expression level of target heterologous nucleotide sequences in plant non-photosynthetic tissues, and obviously enhances the expression tissue specificity of target heterologous nucleotide sequences in plant photosynthetic tissues.