The invention provides a double two-way stop air-operated cylinder valve, which is mainly technically characterized by having a horizontal symmetry structure, adopting a double and two-way stop air operated mode, being directly mounted at openings of cylinders, and mainly comprising cylinder bodies, pistons, push rods, valve seat covers, bearing screw seats, springs, valve cores, valve seats, a conical seal rings, a body, glands and the like, wherein the body is provided with two independent chambers, namely a left chamber and a right chamber in the horizontal direction; the upper end of the body is provided with a quick connect oil connector and a quick connect air connector, and the lower end of the body is provided with two independent channels, namely a left channel and a right channel to switch on and switch off the control air introduced through air source connectors of the cylinder bodies on the left and right sides; and the valve cores are pushed to move through the pistons and the push rods, so that the valve is bidirectionally opened and closed. The double two-way stop air-operated cylinder valve has the advantages of compact structure, high reliability and safety, low cost, convenience for detection and maintenance and the like.