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发明公开
CN102533601A 一株简单芽孢杆菌及其培养方法与应用 失效 - 权利终止

一株简单芽孢杆菌及其培养方法与应用
摘要：
本发明涉及一株简单芽孢杆菌（Bacillussimplex）YM8CGMCCNo.5242及其培养方法与应用。简单芽孢杆菌YM8的培养方法是先将菌种接种于种子培养基中，35℃或55℃、120转/分摇床中培养至菌体浓度达108～109个/mL，然后接种5mL菌液至发酵培养基中，35℃或55℃、120转/分摇床培养。本发明提供的简单芽胞杆菌YM8，可应用于油田的环境治理，具有很好的降解原油的作用，具有较高的研究、应用及市场价值；此外，该菌培养方法简单易操作，生长繁殖迅速，具有扩大化生产的可行性。
摘要（英）：

The invention relates to a bacillus simplex YM8 CGMCC No.5242, and a culture method and application thereof. The culture method for the bacillus simplex YM8 comprises the following steps: inoculating the strain into a seed culture medium; cultivating in a shaking table at the temperature of 35 or 55 DEG C and at the rotating speed of 120 rpm until the strain concentration reaches 10<8> to 10<9>/mL; inoculating 5 mL of bacterial liquid into a culture medium; and cultivating in the shaking table at the temperature of 35 or 55 DEG C and at the rotating speed of 120 rpm. The bacillus simplex YM8 can be applied to environmental management on the oil field, and has a good effect of degrading crude oil, and higher research, application and market values. The culture method is simple and easy to operate, quick in growth and propagation and practicable in expanding production.

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