The invention relates to a magnetic particle chemiluminescent kit for detecting furaltadone metabolites, which comprises the following reagents: a luminescent marker, a fluorescein marker, a standard material, a quality control material, and a separating reagent. The luminescent marker is a furaltadone metabolite semiantigen marked by an isoluminol luminescent marker; the fluorescein marker is a furaltadone metabolite monoclonal antibody marked by a fluorescein or its derivative; the separating reagent is a paramagnetic nanometer micro-bead coated by a sheep anti-FITC monoclonal antibody. The invention also relates to a method for detecting furaltadone metabolites in animal-origin food by using the kit, and the method has high sensitivity, specificity and high detection speed for furaltadone metabolites.