The invention relates to a tissue culture and rapid propagation method for a wine grape variety, which comprises the following steps of: (1) culture medium selecting and making method: selecting GS as a basic culture medium, and adding cane sugar as a sugar source, agar as a coagulating agent and antibiotics as penicillin sodium for injection, wherein the pH value is 5.5-6.0; (2) material selecting and inoculating method: selecting a aseptically cultured aseptic adult grape seedling, scissoring the aseptic seedling stem under aseptic conditions, and inoculating the aseptic seedling stem on the culture medium made in the step (1); and (3) culture environment: controlling the illumination intensity at 4000-6000 LX, and controlling the temperature at 23-25 DEG C. The invention has the characteristic that the obtained wine grape tissue culture seedling is widely popularized and applied to the rapid propagation and planting of wine grape and has the characteristics of: (1) rapid propagation: the propagation period of wine grape can be shortened; and (2) strong root forming ability: the obtained aseptic seedling has developed root system, which is beneficial to transplantation.