The invention discloses a full-automatic air microbe sampler. The sampler comprises a sample collection system and a sample liquid distribution system; wherein the sample collection system comprises a sampling head, a blower, an impact sampling bottle, a liquid storage bottle, an injection pump and a sampling pump; and two outlets of the sampling head are respectively communicated with the blower and the impact sampling bottle; an air inlet of the sampling pump is communicated with the impact sampling bottle; the liquid storage bottle is consequentially communicated with the injection pump and the impact sampling bottle; the sample liquid distribution system comprises a peristaltic pump and an injection device; the peristaltic pump is communicated with the impact sampling bottle, and an outlet of the peristaltic pump is connected with the injection device through a pipeline. According to the full-automatic air microbe sampler, the sampling head can utilize a two-stage virtual impact theory, so that wall damage of particles is reduced, and high particle diameter cutting efficiency is obtained; simultaneously, sampling flow is large, microbial particle collection density is improved, further experimental analysis is facilitated, and sensitivity and accuracy of devices are improved.