The invention belongs to the technical field of agricultural microbe, and particularly to relates to a Haemophilus parasuis 5 type high-density fermentation medium which includes the following components: sodium glutamate 1 to 10g/L, yeast powder 5 to 50 g/L, sodium chloride 1 to 10 g/L, potassium phosphate 0.063 to 2.502g/L, and sanshui dipotassium hydrogen phosphate 0.351 to 14.044g/L as measured according to weight/volume, and includes bovine serum 5 to 40%, NAD 1 to per mille 10, and water. The Haemophilus parasuis 5 type high-density fermentation medium facilitates the high-density fermentation of the Haemophilus parasuis 5 type, the thallus growth speed is quick, more thallus content can be obtained, and the viable count of the cultivated Haemophilus parasuis 5 type amounts to 5 billion. The fermentation medium has a wide material source, is low in cost, can effectively widen and increase the Haemophilus parasuis 5 type and satisfies large vaccine production.