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发明公开
CN102565031A 检测氯霉素的磁颗粒化学发光试剂盒及其应用 无效 - 撤回

检测氯霉素的磁颗粒化学发光试剂盒及其应用
摘要：
本发明涉及检测氯霉素的磁颗粒化学发光试剂盒，包括的试剂有：发光标记物、荧光素标记物、标准品、质控品、分离试剂。发光标记物是异鲁米诺发光标记物标记的氯霉素半抗原，荧光标记物是指荧光素或其衍生物标记的氯霉素单克隆抗体，分离试剂是指包被有羊抗FITC单克隆抗体的顺磁性纳米微珠。本发明还涉及一种采用所述的试剂盒检测动物源性食品中氯霉素的方法，本方法对氯霉素检测具备较高的灵敏度、特异性和较快的检测速度。
摘要（英）：

The invention relates to a magnetic granule chemiluminescence kit for detecting chloramphenicol, which comprises the following reagents: a luminescence marker, a fluorescein marker, a standard preparation, a quality control product and a separation reagent, wherein the luminescence marker is chloramphenicol hapten labeled by an isoluminol luminescence marker; the fluorescent marker is a chloramphenicol monoclonal antibody labeled by fluorescein or a derivative thereof; and the separation reagent is a paramagnetic nano microbead coated with a goat anti-FITC (fluorescein isothiocyanate) monoclonal antibody. The invention also relates to a method for detecting chloramphenicol in animal-derived food by adopting the kit. The method has higher sensitivity, specificity and detection speed when used for detecting chloramphenicol.

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