The invention discloses an efficient garlic test-tube plantlet rooting culture medium, belonging to the technical field of garlic tissue culture, and solves the problem that the conventional test-tube plantlet rooting rate is low. Each liter of the culture medium comprises 1/2MS, 0.5 mg of NAA, 0.1mg of IAA, 60000mg of sucrose and the balance of distilled water in a weight concentration; and the pH value is 5.8-6.0. The efficient garlic test-tube plantlet rooting culture medium is screened out by regulating plant hormones and the sucrose concentration on the basis of a large number of experiments; the rooting rate and the root quality of test-tube plantlets are improved; the rooting rate of the test-tube plantlets is 95%; the transplanting survival ratio is 98.6%; and the culture medium plays a certain role in promoting the formation of test-tube microbulbs and can provide a technical guarantee for breeding virus-free garlic seedlings.