The invention relates to a thermal transfer method of a graphene thin film, which comprises the following steps of: firstly growing the graphene thin film on the surface of a copper foil by utilizing a chemical vapor deposition device; then fixing and reversely buckling one end of the copper coil on the edge of a target substrate, and flatly attaching onto the surface of the target substrate; successively tidily putting the target substrate and the copper coil on a supporting table and slowly pushing into a heat transfer machine roller, and transferring the graphene thin film at one side of the copper foil to the target substrate by utilizing the pressure and the heat of the roller; and finally fixing the movable end of the copper foil, standing and cooling for a period of time, and taking the copper foil down, so that the graphene thin film on the surface of the one side of the copper foil is transferred onto the target substrate. The graphene thermal transfer method provided by the invention has the beneficial effects that the operation is convenient, the process is simple, an organic medium is not added in the whole operation process, the graphene thin film with a size of tens of micrometers or above can be successfully transferred to any target substrate, and the thermal transfer method is suitable for the development, the research and other fields of graphene semiconductor devices.