The invention provides a method for preparing mesoporous titanium dioxide by a template method. The method comprises the following steps that: poly(glycol)-embedded-poly(propylene glycol)-embedded-poly(glycol) is added into water, then, ammonium fluoride and trimethylbenzene are added and are uniformly stirred with the water, and template solution is obtained; tetraethyl orthosilicate and isopropyl titanate are added into the obtained template solution and are uniformly stirred with the obtained template solution, then, the materials are transferred to an autoclave and are heated for 20 to 26 hours at 110 to 130 DEG C, and powder is obtained through filtering and drying; and the obtained powder is added into mixed solution of concentrated nitric acid and hydrogen peroxide, organic matters in the powder are removed through oxidation, and the mesoporous titanium dioxide is obtained. The mesoporous material obtained by adopting the method has the advantages that the bore diameter size distribution range is narrow, and the specific surface area is large; and dye-sensitized solar cells prepared from the mesoporous material have the advantages that the open circuit voltage can reach 0.74 V, the cell efficiency can reach 7.51 percent, and the filling factor can reach 75.7 percent.