The invention discloses a transportation and storage method of fresh tea leaves. The method comprises the following steps: (1) picking fresh tea: manually picking fresh tea leaves from tea trees according to a manner that one sprout is provided one, two or three leaves; (2) centralized storage: placing the picked fresh tea leaves in a container in a centralized manner, which is filled with nitrogen gas in advance; (3) centralizing the fresh tea leaves to the container, then introducing nitrogen gas, and sealing the container; and (4) transportation: transporting the container to a tea plant and storing the container at low temperature. According to the invention, the problems that the fresh tea leaves are severely oxidized due to contact with air during the traditional transportation and storage process, severe oxidative deterioration of fresh tea leaves is caused by high-temperature storage, so that the final tea quality is poor and severe in loss and the like are solved.