The invention provides an anti-fake alcohol bottle and a manufacturing method. The anti-fake alcohol bottle comprises an outer bottle cover and a bottle body, and a one-way valve cover is arranged at a bottle mouth of the bottle body. During production, firstly alcohol is filled in the bottle mouth of the bottle body, then the cooled one-way valve cover is installed in the bottle mouth, and the one-way valve cover is fixedly connected with the bottle mouth in sealing mode. The one-way valve cover is fixedly connected with the bottle mouth in sealing mode by using the thermal expansion and contraction method so as to enable the one-way valve cover to be in interference fit with the bottle mouth at normal temperature, and the one-way valve cover and the bottle mouth are difficult to separate whether lawbreakers adopt warming or cooling and mechanical methods. Due to the fact that the alcohol can be poured out from the one-way valve cover and is difficult to be poured into the bottle through the one-way valve cover, the anti-fake alcohol bottle and the manufacturing method prevent the lawbreakers from reusing used famous brand alcohol bottles.