The invention provides a benthic aquatic animal stereo breeding device which comprises the step of arranging a plurality of mesh cloth pieces in a water body in a manner of having a certain angle with the bottom part of the water body, so that an inclined angle formed between the mesh cloth pieces and the bottom part of the water body is 30-60 degrees; and the vertical height of the mesh cloth pieces relative to the bottom part of the water body is larger than 50 percent of depth of the water body. The benthic aquatic animal three-dimensional breeding technology has the beneficial effects that the stereo breeding of benthic aquatic animals in the water body is realized, the utilization rate of a breeding space is improved, the breeding density is increased, the high yield production is realized, and the bait pollution is reduced; meanwhile, poisonous gas generated by residual baits and excrements is reduced, and the harm of the poisonous gas to the benthic aquatic animals is reduced; and the operation is simple, and the popularization is easy.