The invention relates to a negative-ion ultra-clean edible fungus inoculation hood. The negative-ion ultra-clean edible fungus inoculation hood comprises an outer box body, an inner box body, an alternating current-direct current converter, a storage battery, a direct current axial flow fan, an ultraviolet lamp, filter paper, a non-woven fabrics, a stainless steel net, an up-and-down sliding door, a power supply switch and an inner box work bench, wherein channels are machined at the two sides of the outer box body and the inner box body; active carbon is filled inside the channels; the work bench is arranged at the bottom part of the inner box body; front and rear sides and the wall of the outer box body are fixed and sealed in a welding manner; the axial flow fan is arranged on the back side of an upper cover of the inner box body; the stainless steel net is arranged in the inner box body, and the filter paper and the non-woven fabrics are paved, and a plurality of air holes are arranged on the base plate of the outer box body. According to the device, the inoculation environment is purified; the pollution rate of mixed bacteria is reduced, and the inoculation environment reaches 99.99% of an ultra-purified environment, and therefore, the growth of edible fungus mycelium can be speeded up; the production period can be shortened and the yield of the edible fungus is improved.