The invention relates to a method for breeding white flammulina velutipes through three monosporous hybridization. The method comprises parent selection, monospore isolation, first round of hybridization and screening, identification of hybrid progeny F1, second round of hybridization and screening and identification of hybrid progeny F2. The method has the following advantages that a yellow flammulina velutipes and two flammulina velutipes are taken as the parents and the hybrid progenies are the white flammulina velutipes; the white flammulina velutipes inherits such excellent traits of the parent yellow flammulina velutipes as high yield and prematurity and has the advantages of high yield, stable agronomic traits, good merchandise traits and the like; and the method is a good one for breeding the white flammulina velutipes through hybridization.