The invention relates to feed, and in particular relates to powdered oil feed and a preparation method thereof. The invention mainly solves the technical problems that the powdered oil in the prior art is easy to be fused, oxidized and deteriorated and is difficult to store, main nutritional ingredient of the powdered oil is fat, content of other nutritional ingredient is low and the powdered oil can be used as energy feed only in animal feed. The powdered oil feed disclosed by the invention comprises animal oil, plant oil, animal protein, plant protein, minerals, trace elements and vitamins. The preparation method disclosed by the invention comprises the following steps of: mixing the animal oil, plant oil, animal protein, plant protein and emulsifier and emulsifying at high speed; then carrying out high pressure homogenization; and carrying out instant variable frequency spray drying, adding the minerals, trace elements and vitamins and mixing; and finally packaging to obtain the finished product lipopeptid amino acid protein.