The invention discloses a preparation method of a fresh and hot flavor seasoning and a seasoning prepared by using the preparation method. The preparation method comprises the steps of: crushing monosodium glutamate and edible salt; crushing spice; washing and pretreating vegetables; mixing and processing the pretreated vegetables and the spice with a proportion being 1: (0.01-0.1); mixing and stirring the processed vegetables, the spice, the crushed monosodium glutamate and the edible salt, and an additive with a proportion being 1:(0.1-0.6):(0.02-0.08) to obtain a liquid material; making the liquid material into uniform granular materials through a granulating machine; drying and sterilizing the prepared granular materials by using a drying and sterilizing device; and selecting the dried and sterilized granular materials by adopting a selecting device. The fresh and hot flavor seasoning prepared by adopting the preparation method is fresh and hot in taste and is mellow in mouth feel, greatly meets the demand on variety of the seasoning by a consumer, and is suitable for massive industrialized application.