The invention relates to a novel high-efficient testing and regulating process for separate injection wells, which belongs to the oil field testing field, is capable of reducing test cost and guaranteeing high test success rate, and mainly utilizes a ground controller 1, a fiber-optic cable controller 2, a cable car 3, an optical fiber cable 4, an oil pipe 5, a downhole testing and regulating apparatus 6, a bridge eccentric water distributor 7 and packers 8. The downhole testing and regulating apparatus 6 extends downwards into the bridge type eccentric water distributor 7 via the optical fiber cable 4. The ground controller 1 is used for controlling the testing and regulating process. The novel high-efficient testing and regulating process utilizing the optical fiber cable is fully applicable to wells with complex pressures and has the advantages of low cost, high testing and regulating efficiency, convenience in use, accurate regulating result and capability of guaranteeing water injection qualification rate of the water injection wells.