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CN102636380A 一种农药残留检测的样品分离与提取方法 无效 - 撤回

  • 专利标题： 一种农药残留检测的样品分离与提取方法
  • 专利标题（英）： Method for separating and extracting samples for pesticide residue detection
  • 申请号： CN201210088857.4
    申请日： 2012-03-30
  • 公开(公告)号： CN102636380A
    公开(公告)日： 2012-08-15
  • 发明人: 孙霞 , 王相友 , 刘君峰 , 杜淑媛 , 李青青
  • 申请人： 山东理工大学
  • 申请人地址： 山东省淄博市高新技术产业开发区高创园D座1012室
  • 专利权人： 山东理工大学
  • 当前专利权人： 山东理工大学
  • 当前专利权人地址： 山东省淄博市高新技术产业开发区高创园D座1012室
  • 主分类号： G01N1/34
  • IPC分类号： G01N1/34
一种农药残留检测的样品分离与提取方法
摘要：
本发明涉及一种农药残留检测的样品分离与提取方法，其特征在于：分离过程共分为四个部分，具体过程如下：25μL30nm的裹着生物亲和素的Fe3O4纳米颗粒水溶液放入含250μL磷酸盐缓冲液（PBS，10mM，pH7.4）的离心管中，将离心管放入磁分离器中施磁30分钟，去掉废液。再将150μL的PBS和75μL浓度为100μg/mL的连接生物素的抗体放入离心管中，在15r/min的混匀器中混匀30min，再施磁30min，去掉废液。将蔬菜水果榨汁后，用滤纸过滤后，取200μL的样品液加入离心管中。在混匀器中混匀30min，然后施磁30min，去掉废液。加入150μL的水，施磁30min，去掉废液，然后加入60μL的水等待测试。
摘要（英）：

The invention relates to a method for separating and extracting samples for pesticide residue detection. The method is characterized in that a separation process is divided into four portions and particularly includes placing 25 micro-liters of 30nm Fe3O4 nano-particle aqueous solution containing biological avidin into a centrifugal tube containing 250 micro-liters of phosphate buffer solution (PBS) with the volume of 10mM and the pH (potential of hydrogen) of 7.4, placing the centrifugal tube into a magnetic separator to be magnetized for 30 minutes, and removing waste liquor; then placing 150 micro-liters of PBS and 75 micro-liters of biotin connecting antibody with the concentration of 100 micro-grams/mL into the centrifugal tube, uniformly mixing in a 15r/min mixer for 30 minutes, magnetizing mixture for 30 minutes, and removing waste liquor; filtering juice by a filter after vegetables and fruits are juiced, and adding 200 micro-liters of sample liquor into the centrifugal tube; uniformly mixing the sample liquor with the mixture in the mixer for 30 minutes, magnetizing for 30 minutes and removing waste liquor; and adding 150 micro-liters of water, magnetizing for 30 minutes, removing waste liquor, then adding 60 micro-liters of water and standing for being tested.

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