The invention provides a recombinant rotavirus VP6 carrier protein and the preparation thereof. Six restriction enzyme loci used for the insertion of exogenous epitope genes are on a recombinant rotavirus VP6 carrier protein encoding gene, the original molecular structure of the VP6 protein is not affected, all the original epitopes of the VP6 protein are retained, all the epitopes are positioned on the surface of a protein molecule, and the exogenous epitope immunology characteristic is shown to the maximum extent, so that the epitopes of other viruses are inserted into the recombinant rotavirus VP6 carrier protein disclosed by the invention, and jointly recombinant chimeric vaccine and reagent of rotavirus RV and other viruses are constructed.