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CN102657069A 一种团集刚毛藻培养液及其制备方法 无效 - 撤回

  • 专利标题： 一种团集刚毛藻培养液及其制备方法
  • 专利标题（英）： Cladophora glomerata culture solution and preparation method thereof
  • 申请号： CN201210140368.9
    申请日： 2012-05-08
  • 公开(公告)号： CN102657069A
    公开(公告)日： 2012-09-12
  • 发明人: 石瑛 , 胡变芳 , 李砧 , 张猛 , 李艳晖 , 张锦华 , 乔凯
  • 申请人： 太原师范学院
  • 申请人地址： 山西省太原市南内环街189号
  • 专利权人： 太原师范学院
  • 当前专利权人： 太原师范学院
  • 当前专利权人地址： 山西省太原市南内环街189号
  • 主分类号： A01G33/00
  • IPC分类号： A01G33/00
一种团集刚毛藻培养液及其制备方法
摘要：
本发明涉及一种团集刚毛藻培养液及其制备方法，其中每1000m1水中含有MgSO4·7H2O 300-350mg、CaCl2 300-400mg、KNO3 3.0-4.0mg、NaHCO3 150-170mg、KH2PO4 5.0-6.0mg、葡萄糖20g，用NaHCO3调节pH值为8.2-8.45。实验室培养条件：温度18-19℃，光照日夜比12h∶12h，强度2500-4500LX。规模培养条件：温度18-19℃，光照日夜比12h∶12h，强度2500-4500LX，流速20-100cm/s。本发明能够促进团集刚毛藻生长，所用生产设备和生产工艺简单，成本低廉，适合保种及大规模培养团集刚毛藻。
摘要（英）：

The invention relates to cladophora glomerata culture solution and a preparation method thereof. The cladophora glomerata culture solution comprises 300mg to 350mg of MgSO4.7H2O, 300mg to 400mg of CaCl2, 3.0mg to 4.0mg of KNO3, 150mg to 170mg of NaHCO3, 5.0mg to 6.0mg of KH2PO4 and 20g of glucose contained in 1000ml of water, and the pH value of the solution is regulated to 8.2 to 8.45 by virtue of the NaHCO3. The laboratory culture conditions are that the temperature ranges from 18 to 19 DEG C, the ratio of illuminance day and night equals to 12h to 12h, and the intensity ranges from 2500LX to 4500LX. The scale culture conditions are that the temperature ranges from 18 to 19 DEG C, the ratio of illuminance day and night equals to 12h to 12h, the intensity ranges from 2500LX to 4500LX, and the flow rate ranges from 20cm/s to 100cm/s. According to the invention, the growth of cladophora glomerata is promoted, production equipment and production technology are simple, and the cost is low, and therefore the cladophora glomerata culture solution is suitable for the preservation and the large-scale culture of the cladophora glomerata.

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