The invention discloses a dynamic characteristics calibration method of a current/frequency conversion circuit, wherein two multifunctional calibration sources are used for providing direct current signal input and alternating current signal input, and a high-speed pulse data collection circuit is adopted to achieve calibration of dynamic characteristics. Devices for calibration include a multifunctional calibration source A, a multifunctional calibration source B, the high-speed pulse data collection circuit, a communication board and an industrial personal computer. The multifunctional calibration source A provides direct current input for the current/frequency conversion circuit to serve as bias. The multifunctional calibration source B provides alternating current input for the current/frequency conversion circuit to serve as dynamic input. A high-speed pulse sampling circuit conducts high-speed multi-point collection for output pulse to achieve conversion of pulse signals to pulse number. The communication board serves as a port of the high-speed pulse data collection circuit and the industrial personal computer and uploads the pulse number to the industrial personal computer. The industrial personal computer conducts storage, interpolation processing and waveform display for the data. The dynamic characteristics calibration method solves the problem of conversion bandwidth calibration of the current/frequency conversion circuit.