The invention discloses a prefabricated frame structure connection supporting template with adjustable vertical height. The prefabricated frame structure connection supporting template comprises a prefabricated column fixed supporting device and a prefabricated beam connecting template device, wherein the prefabricated beam connecting template device is positioned on the upper side of the prefabricated column fixed supporting device and is connected with the prefabricated column fixed supporting device; the prefabricated column fixed supporting device comprises a connection supporting component and a first prefabricated column fixing plate; the connection supporting component comprises a second prefabricated column fixing plate, a horizontal supporting plate and a triangular supporting plate; and the prefabricated beam connecting template device comprises a beam bottom template and two opposite beam side templates which are positioned on the upper side of the beam bottom template. By the prefabricated frame structure connection supporting template, the horizontal position and the vertical height of a prefabricated beam can be conveniently positioned, the mounting efficiency is high, and the beam column connection construction speed of a prefabricated assembled type frame structure is directly increased; and the prefabricated frame structure connection supporting template can be recycled, and has remarkable economic benefit.