The invention discloses a method for detoxifying a clonal tissue culture explant of Camellia oleifera Abel. The method comprises the following steps of: (1) pre-treating the clonal tissue culture explant of the Camellia oleifera Abel at low temperature; and (2) disinfecting the clonal tissue culture explant, which is pre-treated at low temperature, of the Camellia oleifera Abel by using ethanol, washing, and treating by using HgCl2, wherein the HgCl2 treatment is that the clonal tissue culture explant of the Camellia oleifera Abel is intermittently treated for over two times, the total time of the HgCl2 treatment is 4 to 20 minutes, and the clonal tissue culture explant of the Camellia oleifera Abel is washed by using water after treated by using HgCl2 every time. The method is easy to operate and low in pollution rate; and the technical problem that the traditional preservation rate is low is solved.