The invention relates to an MAAPN (Muscular Amino Acids and Peptides and Nucleosides) extractive containing drug regimen ingredients such as polypeptide, amino acid, nucleoside and nucleotide, as well as the composition thereof. The preparation method comprises the following steps: taking out heart and muscle of a rabbit, removing grease, homogenate, vacuum-pumping, leading in carbon dioxide, then vacuum-pumping, and inflating repeatedly for two times; respectively adding carbon dioxide saturated rabbit heart and muscle slurry into liquid carbon dioxide for stirring, cooling down to 80 DEG C below zero, keeping for 6 to 10 hours, increasing temperature to 40 to 50 DEG C, respectively taking rabbit heart and muscle after freeze thawing according weight ratio of one to three and adding into injection water respectively, adding heart tissue solution into elastic protease, and adding muscular tissue solution into papayotin; combining zymolytic heart tissue solution and muscular tissue solution, carrying out centrifugation, taking supernatant fluid, adding 0.3 times of activated charcoal in weight, stirring for 30 min in the room temperature, and filtering through a micropore filtration film. Excipient can be added into the composition prepared by the extractive so as to make squirt, powder for injection or freeze-dried powder.