The invention discloses a physiological identification method for species property resistance and expression resistance of tea trees, comprising the following steps: when tea trees are at a critical dormancy state at 10-13 degrees centigrade, selecting tea tree branches which are not pruned and which have larger leaves insertion amount; collecting the first leaves with no plant diseases and insect pests on lignification parts from up to down, after taking back to laboratories, carrying out detection for basic values of species property resistance indexes and expression resistance indexes; then measuring species property resistance indexes and expression resistance indexes of tea trees again when temperature is reduced to below 0 degree centigrade, taking detection values at two times as longitudinal coordinates and taking temperature as horizontal coordinates to draw a plane linear line; and judging resistance is weak or strong according to slope of the linear line. The method provided by the invention reduces human observation error under a condition that tea trees naturally grow out of doors at low temperature, avoids resistance performance differences caused by factors such as climate, geographical environment conditions, management levels and so on, and evaluates cold resistance of tea trees according to species property resistance and expression resistance even if not needing to wait for extreme minimum temperature so as to greatly shorten identification cycle.