The invention discloses a left-handed and right-handed rotation nut locking device for a large-diameter shaft end, which includes a large-diameter shaft lever (1), a clamped body (2) and a locking device, wherein left-handed and right-handed rotation screw threads are arranged at an E section and an F section of an end part of the large-diameter shaft lever (1); the locking device comprises fixing nuts (3), tensioning bolts (4), pushing bolts (6) and locking nuts (5); the fixing nuts (3) are screwed tightly at the E section screw thread; the locking nuts (5) are screwed tightly at the F section screw thread; the distances between the fixing nuts (3) and the locking nuts (5) range from 0.5 mm to 20 mm; and the fixing nuts (3) and the locking nuts (5) are connected by the tensioning bolts (4) and the pushing bolts (6) respectively. The large-diameter shaft end left-handed and right-handed rotation nut locking device is suitable for fastening the large-diameter shaft end.