The invention relates to a preparation method for a ferment type fruit tobacco flavor, comprising the following steps: uniformly mixing an apple juice and a banana juice in a weight ratio of 1:0.5-2 to obtain a complex fruit juice; adding a sugar and an acid into the complex fruit juice and adjusting a sugar degree and an acid degree of the fruit juice to ensure a sugar content of 11 DEG C to 12 DEG C and a acid degree of 3.5 to4.0 in the complex fruit juice; adding an active saccharomyces cerevisiae into the obtained complex fruit juice in a mass ratio of the juice to the active saccharomyces cerevisiae being 1000-100 : 1 and uniformly mixing the obtained product for alcoholic fermentation; after the fermentation, filtering out the saccharomyces cerevisiae, subjecting the obtained product to acetic fermentation in an acetic fermentation tank; and filtering the fermented fruit juice and storing the obtained product in an airtight container. The method, through microbial fermentation, can increase a content of various aroma materials in the fruit juice and is of great importance for cigarette aroma improvement. Adding the tobacco flavor produced through the method into cigarettes can lead to rich cigarette smoke and aroma, with taste of the cigarettes obviously improved.