The invention discloses a sterilizing composition containing js399-19 and an amide fungicide. The sterilizing composition comprises a first effective active ingredient js399-19 and a second effective active ingredient amide fungicide selected from silthiopham, isotianil, isopyrazam, fluxapyroxad and fluopyram. The weight percentage of the first and second active ingredients is 1:20-20:1, preferably 1:10-10:1. The composition can be used to formulate agriculturally permitted water dispersible granule, wettable powder, suspending agents, flowable concentrates for seed coating, dispersible powder for seed treatment or dispersible granule for seed treatment. The sterilizing composition is reasonable in components, good in sterilizing effect and low in cost, and the activity and sterilizing effect are not simply overlapped by components. Compared with current single preparation, besides the remarkable sterilizing effect, the sterilizing composition also has a remarkable synergetic effect, has no pollution to crops, is good in safety, and meets the safety requirement of pesticide preparations. The sterilizing composition containing js399-19 and the amide fungicide provided by the invention has remarkable preventing and treating effect on take-all disease of wheat and rust disease.