The invention relates to an industrial production method of bacillus natto. The industrial production method includes steps of sterilizing a culture room; preparing a culture medium and sterilizing the medium under high pressure; pouring the culture medium into trays before being solidified; arranging and placing the trays on culture racks uniformly; uniformly brushing prepared stock seeds on surfaces of the solidified culture medium in the trays with brushes which are sterilized under high pressure; placing the trays holding the culture medium brushed with the stock seeds on the culture racks; scraping lawn with sterilized flat scrapers after the trays are overgrown with the white lawn in 24 hours; uniformly mixing the lawn with sterilized starch according to a ratio of 1kg:2kg; and then placing the mixture of the lawn and the sterilized starch into a freeze dryer to dry thalli so as to obtain the bacillus natto finally. The industrial production method has the advantage of capabilities of saving energy, realizing industrial production, reducing production cost greatly and improving product quality.