The invention discloses a method for floating and separating copper and nickel from high nickel matte and relates to the method for sorting copper concentrate and nickel concentrate from high nickel matte by using a flotation column. The method is characterized in that the flotation column is adopted to perform a flotation process. According to the method for floating and separating the copper and the nickel from the high nickel matte, the flotation column is applied to all the production flow of rough flotation, scavenging flotation and precise flotation, so that the automation degree is high; a proper technical condition is selected, so that the technical flow is short and the operation is convenient to perform; the equipment is little; the operation cost is low; and the technology is reliable and convenient to perform.