The invention relates to an overwintering preserving breeding method for an aphidius gifuensis and belongs to the technical field of biological control. The overwintering preserving breeding method for the aphidius gifuensis solves the problem that in the prior art, overwintering preservation is performed on the aphidius gifuensis difficultly, and the heavy demand for the aphidius gifuensis in a tobacco planting season in a coming year is difficult to meet. The overwintering preserving breeding method comprises a first step of greenhouse aphidius gifuensis selection which comprises cultivation of host plants, collecting of an aphidius gifuensis source, aphidius gifuensis inoculation in a greenhouse, and purification and rejuvenation; a second step of winter greenhouse aphidius gifuensis preservation which comprises cultivation of aphidius gifuensis preserving plants, aphidius gifuensis inoculation in the greenhouse, and purification and rejuvenation; and a third step of expanding propagation in the greenhouse. The aphidius gifuensis is successfully accumulated and preserved during the time from finish of massive reproduction of the aphidius gifuensis in August to April in a coming year and before massive reproduction of the aphidius gifuensis, and the aphidius gifuensises with enough quantity and good quality are provided for massive reproduction of the aphidius gifuensis in a next year.