The invention provides a method for removing calcium and magnesium in aloe gel juice, which comprises the following steps of: after cleaning aloe leaves, peeling off the aloe leaves; grinding and squeezing; sterilizing; carrying out enzymolysis, filtering, resterilizing and cooling; decoloring by macroporous anion resin; removing the calcium and the magnesium by strong-acid cation-exchange resin;and removing sulfate radicals and chloride ions by weak-base anion resin. The method has a high removal rate; the obtained aloe gel juice does not contain precipitates and is transparent and clear; the removal of other ingredients is not influenced; and the nutritional ingredients of the aloe product are also not influenced. The method is beneficial for protecting the environment, the resin can be repeatedly used for times after being regenerated, and the production cost is controllable.