The invention discloses a misoperation-preventive locking logic automatic test system for an intelligent transformer substation, comprising a data layer, a business layer and a presentation layer, wherein the data layer comprises a GOOSE (Generic Object Oriented Substation Event) message receiving and sending module for receiving a GOOSE signal, a switch and disconnecting link deflection signal and a switching value signal from a monitoring system of the intelligent transformer substation and sending the received data to a comparison and analysis module of the business layer, and an SCD (Science Citation Database) file parsing module for analyzing the GOOSE signal from the system of the intelligent transformer substation; and the business layer comprises a standard library for storing an input misoperation-preventive locking logic database, and the comparison and analysis module used for performing real-time logical operation by different switch and disconnecting link position combinations received from the GOOSE message receiving and sending module according to the input misoperation-preventive locking logic database, comparing the standard output result with the locking result fed back by a monitoring device and verifying validity of the misoperation-preventive locking logic of the monitoring device. The system can verify the validity and integrity of the misoperation-preventive locking logic of the monitoring device.