The invention provides a clock delay control method based on a printed circuit board. The method includes the steps: firstly, setting up a shunt circuit in the printed circuit board design; secondly, extracting a topological structure of the shunt circuit; thirdly, setting a laminated structure in the printed circuit board design; fourthly, adding pulse excitation at a source end and extracting waveforms; fifthly, adding periodic excitation at the source end and extracting waveforms; sixthly, acquiring constant delay waveforms. The clock delay control method based on the printed circuit board is used for realizing high-precision data sampling by a clock, and belongs to innovation on high-precision data acquisition systems. Multichannel signals are subjected to board delay control by means of a characteristic of constant delay of signals transmitted in media, the delay control precision can reach a ps level, and data sampling precision by the clock is improved by controlling delay of a group of clock signals.