The invention provides a snakegourd peel injection formulation and a preparation method of the snakegourd peel injection formulation, and belongs to the technical field of medicines. A preparation method of a snakegourd peel extract comprises the following steps of: crushing snakegourd peels into snakegourd peel powder with particle diameters being less than and equal to 250 Mum; adding the powder into water, extracting under certain pressure, removing the pressure, filtering, and then concentrating to obtain a first extractum; enabling the first extractum to pass through a semipermeable membrane, collecting retention liquid, reacting with a mixed solution of chloroform and n-butyl alcohol to remove proteins, collecting a water layer, and concentrating to obtain a second extractum; and adding the second extractum into ethanol, standing, centrifuging, taking supernate, concentrating to obtain a third extractum, filtering and then collecting a filtrate. The preparation method is high in working efficiency and low in preparation cost, and the injection formulation prepared from an extracting solution obtained by the method is stable in quality and high in safety.