The invention discloses a carrier for cell culturing. The carrier is a three-dimensional porous graphene support which is fixed on a transparent polystyrene or glass material substrate, liquid tank walls are fixed on the periphery of the three-dimensional porous graphene support to form a culturing tank used for cell culturing. The invention further discloses a preparation method of the carrier for cell culturing. Compared with the prior art, the method includes adopting the three-dimensional porous graphene support infiltrated by solvent before removing a metal catalyst formwork, and removing the air absorbed on the surface of the support by means of vacuum air suction, so that floating of materials on the surface of liquid due to surface tension and density is avoided, and residues of metal and ions can be removed more easily in the process of subsequent treatment. The three-dimensional porous graphene support is used as culturing carrier, realizes three-dimensional culturing of cells, simulates in-vivo growth environment of the cells, is beneficial to maintaining growth states and activity of the cells and accelerates cell growth.