The present invention relates to two methods for calibrating frequency of a magnetic resonance device in a volume segment, both comprising the steps of: using an HF pulse (25) to excite the volume segment, collecting a plurality of echo signals (E1-E5) in different times after excitation, and determining respective frequency spectrum information for each echo signal (E1-E5). Further, the first method comprises the steps of: determining at least one peak in the frequency spectrum information of the echo signals (E1-E5), determining relaxation time for each peak, determining a material for the determined relaxation time for each peak, and performing frequency setting based on instruments according to the resonance frequency of at least one material. The second method comprises the steps of: collecting a plurality of peaks in the frequency spectrum information of the echo signals (E1-E5), determining one material for each peak according to the resonance frequency difference of a special material caused by chemical moving, and performing frequency setting based on instruments according to the measured resonance frequency of the material.