The invention aims at further improving magnetic marking and magnetofection efficiency of magnetic nanoparticles against cells, and designs and constructs a novel cell magnetic marking instrument. The novel cell magnetic marking instrument is a symmetrical rotation magnetic field system, namely that strip-shaped permanent magnets (neodymium iron boron) are symmetrically arranged at the upper part and the lower part, then the magnetic field system becomes a double-layer structure, a cell culture plate slot is arranged at the middle part, the upper-layer magnetic field and the lower-layer magnetic field can interact, the lower magnetic field can drive the upper magnetic field to rotate through magnetic attraction, the acting magnetic field is strengthened and evened, the formed rotating magnetic field enhances the effects of the magnetic nanoparticles against the cells in varying space and time, and the current problem of low efficiency of magnetic marking and magnetofection equipment is solved.