The invention belongs to a kit for quickly performing molecular subtyping determining to salmonella, and particularly relates to the technology of utilizing an F0F1-ATPase molecular motor biosensor Chro-invA, Chro-hut, Chro-spvR and Chro-sdf in the kit to detect specific gene invA and hut of the salmonella, toxic plasmid gene spvR and salmonella enteritidis strain specific gene sdf, so as to determine whether the salmonella contains toxic plasmid and whether the salmonella is the salmonella enteritidis in a subtyping way. The kit has functions that 1, the invA and the hut of the salmonella isdetected, so as to identify target bacterium; 2, the spvR can be detected to determine whether the target bacterium carries the toxic plasmid; and 3, the sdf is detected to determine whether the target bacterium is the salmonella enteritidis. The method can be used for establishing the F0F1-ATPase molecular motor biosensor in the kit, carrying out molecular subtyping of the F0F1-ATPase molecular motor biosensor, and determining whether the salmonella contains the toxic plasmid and whether the salmonella is the salmonella enteritidis in a subtyping way according to a subtyping result. The method has the advantages of being high in sensitivity, fast, simple to operate, and low in cost.