The invention discloses a preparation method of burley tobacco shreds for cigarettes. The preparation method comprises the following steps of: firstly soaking the burley tobacco shreds by using a soaking solution, then removing the solution, drying, cutting the burley tobacco shreds into slices, and finally drying and puffing. The soaking solution is prepared from natural plant fruit and amino acids by adopting the following steps of: extracting the natural plant fruit by using 5-10 times by weight of edible alcohol with the mass concentration of 55-65%, then concentrating the extracting solution into extractum with the relative density of 1.1-1.5, adding the amino acids according to 0.5-3% of the weight of the extractum, and finally diluting with water into 5-20 times. The natural plant fruit is selected from one or more of grapes, cherries and apples. The burley tobacco shreds prepared by the method have high smoking quality reflected in the terms that offensive odor and irritation are reduced and oral cavity is clean; and the burley tobacco shreds can be used for various cigarettes and have wide application range.