The invention relates to an anion chromatographic column and a preparation method thereof, in particular to a preparation method of a carbon nano tube latex agglomeration type anion chromatographic packing. The preparation method takes polystyrene-divinyl benzene as a matrix. The preparation method comprises the steps of preparing a monodisperse linear polystyrene microsphere seed through a dispersion polymerization method, activating the monodisperse linear polystyrene microsphere seed, synthesizing the monodisperse linear polystyrene microsphere seed into a polystyrene-divinyl benzene microsphere through a one-step seed swelling method, and performing extraction to remove pore-foaming agents; preparing carbon nano tube latex with methylamine and 1,4-butanediol diglycidyl ether serving as raw materials through a one-step synthesis method; and agglomerating a layer of carbon nano tube latex on the surface of a sulphonation polystyrene-divinyl benzene base ball through an ionic bonding mode to serve as an anion exchange functional group. The preparation method is low in cost and simple in process. The prepared packing is epigranular, narrow in particle size distribution, good in chemical stability and capable of tolerating a wide pH value range, and does not need screening.