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发明公开
CN102936280A 禽蛋中卵黄高磷蛋白的提取及纯化方法 无效 - 撤回

禽蛋中卵黄高磷蛋白的提取及纯化方法
摘要：
本发明公开了一种禽蛋中卵黄高磷蛋白的提取及纯化方法，而提供一种可直接在食品医药等纯度要求较高的行业应用，所得产品纯度高，操作简单，易于产业化生产的禽蛋中卵黄高磷蛋白的提取及纯化方法。包括如下步骤：取禽蛋中的卵黄去除水溶性物质，采用乙醇脱脂后，用高浓度盐溶液浸提得到卵黄高磷蛋白粗品；利用阴离子交换层析法纯化上述卵黄高磷蛋白粗品并除盐，得到高纯度的卵黄高磷蛋白。本发明的方法采用阴离子交换树脂纯化卵黄高磷蛋白，大幅提高了卵黄高磷蛋白的纯度，而且其回收率也较高。本工艺各环节操作简单，设备成本低廉，易于实现产业化生产，所得卵黄高磷蛋白可直接用于食品医药行业。
摘要（英）：

The invention discloses an extraction and purification method of phosvitin in eggs, which can be directly applied to foods, drugs and other industries with higher requirement on purity, produces products with high purity, is easy to operate and produce industrially. The method comprises the following steps of: removing water-soluble substances from yolk in an egg; degreasing with ethanol; extracting with high-concentration salt liquid to obtain a phosvitin coarse product; purifying the phosvitin coarse product by utilizing an anion exchange chromatography, and removing salt to obtain high-purity phosvitin. According to the method, the phosvitin is purified by adopting the anion exchange chromatography, so that the purity of the phosvitin can be greatly improved, and the reclamation of the phosvitin is high. Each step in the process is easy to operate, the equipment is low in cost, industrial production can be realized easily, and the phosvitin can be directly applied to the industry of foods and drugs.

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