The invention relates to a medicine composition for preventing and treating chicken coccidiosis and a preparation method and application thereof and a premix, belonging to the fields of veterinary medicines and premixes. The medicine composition contains active ingredients prepared from antifeverile dichroa root, sweet wormwood herb, lightyellow sophora root, ash bark, smoked plum, garden burnet root and decoquinate. The medicine composition is suitable for preventing and treating chicken coccidiosis. The medicine composition has the characteristics of definite curative effect, stable preparation, simple preparation process, suitability for industrial production and the like. The medicine composition can be also added to feeds and is used for preparing poultry feeds for promoting growth, such as a premix, a batch and the like.